rabbit polyclonal antibody against rac1  (Bioss)

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Overall survival and the expression levels of Rho/Rac family genes in DLBCL. Kaplan–Meier curve of overall survival for individual member ( A ) RAC1, ( B ) RAC2, ( C ) RND1, ( D ) CDC42, ( E ) RHOQ, ( F ) RHOF; HR, hazard ratio.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Correlation between the expression of Rho/Rac family members’ and BTK. ( A ) Co-expression heatmap of Rho/Rac family genes and BTK; ( B ) Scatter diagram demonstrated the correlation between BTK and RAC1 , ( C ) CDC42, ( D ) RHOF and ( E ) RHOQ. * p <0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: RAC1 expression levels in 18 different types of tumor tissues and paired adjacent normal tissues. * p < 0.05; ** p < 0.01; *** p < 0.001; ns: non-significance. BLCA, Bladder Urothelial Carcinoma; BRCA, Breast invasive carcinoma; CHOL, Cholangio carcinoma; COAD, Colon adenocarcinoma; ESCA, Esophageal carcinoma; HNSC, Head and Neck squamous cell carcinoma; KICH, Kidney Chromophobe; KIRC, Kidney renal clear cell carcinoma; KIRP, Kidney renal papillary cell carcinoma; LIHC, Liver hepatocellular carcinoma; LUAD, Lung adenocarcinoma; LUSC, Lung squamous cell carcinoma; PAAD, Pancreatic adenocarcinoma; PRAD, Prostate adenocarcinoma; READ, Rectum adenocarcinoma; STAD, Stomach adenocarcinoma; THCA, Thyroid carcinoma; UCEC, Uterine Corpus Endometrial Carcinoma.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Correlation between RAC1 expression and DLBCL clinical characteristics.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Logistic analyses of RAC1 expression and clinical characteristics of DLBCL.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: RAC1 expression in patients with DLBCL according to different clinical stages.( A ) In the TCGA-DLBC dataset, RAC1 was upregulated in stage I/II as compared with that in stage III/IV. ( B ) RAC1 protein IHC staining in lymphadenitis and ( C ) DLBCL stage II, ( D ) stage III, ( E ) stage IV. Magnification 20 × 20, * p < 0.05. The staining of 3,3′-diaminobenzidine (DAB) was employed for RAC1 expression (yellow and brown) compared to negative sample (blue). Scale bar (bottom, right) = 100 μm.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Cox regression analyses of clinical features associated with DLBCL overall survival.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques:

Journal: Frontiers in Oncology

Article Title: The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma

doi: 10.3389/fonc.2024.1347270

Figure Lengend Snippet: The correlation of integrin αvβ6 expression and RAC1 expression with clinicopathologic variables in cases of gastric cancer.

Article Snippet: Rabbit polyclonal antibody against ITGB6 (dilution, 1:500; #28378-1-AP; Proteintech; USA) and Rabbit polyclonal antibody against Rac1 (dilution, 1:500; #24072-1-AP; Proteintech; USA) were utilized.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma

doi: 10.3389/fonc.2024.1347270

Figure Lengend Snippet: The manifestation of ITGB6 and Rac1 in diverse specimens. (A) Immunohistochemical staining exhibited diminished ITGB6 expression in adjacent normal tissues. (B) Immunohistochemical staining revealed elevated ITGB6 expression in tumor tissues. (C) ITGB6 expression in tumor tissues lacking lymphatic invasion. (D) ITGB6 expression in tumor tissues with lymphatic invasion. (E) Immunohistochemical staining displayed reduced Rac1 expression in adjacent normal tissues. (F) Immunohistochemical staining exhibited heightened Rac1 expression in tumor tissues. (G) Rac1 expression in tumor tissues without lymphatic invasion. (H) Rac1 expression in tumor tissues with lymphatic invasion.

Article Snippet: Rabbit polyclonal antibody against ITGB6 (dilution, 1:500; #28378-1-AP; Proteintech; USA) and Rabbit polyclonal antibody against Rac1 (dilution, 1:500; #24072-1-AP; Proteintech; USA) were utilized.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Frontiers in Oncology

Article Title: The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma

doi: 10.3389/fonc.2024.1347270

Figure Lengend Snippet: (A) The level of ITGB6 expression was assessed using the H-score in both adjacent normal tissue specimens and tumor tissues. (B) The H-score of ITGB6 expression was evaluated in adjacent normal tissue specimens, comparing those with lymph node metastasis and those without. (C) We measured the H-score of Rac1 expression in both adjacent normal tissue specimens and tumor tissues. (D) The H-score of Rac1 expression was analyzed in adjacent normal tissue specimens, comparing those with lymph node metastasis and those without. *P<0.05; **P<0.001; ***P<0.0001.

Article Snippet: Rabbit polyclonal antibody against ITGB6 (dilution, 1:500; #28378-1-AP; Proteintech; USA) and Rabbit polyclonal antibody against Rac1 (dilution, 1:500; #24072-1-AP; Proteintech; USA) were utilized.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma

doi: 10.3389/fonc.2024.1347270

Figure Lengend Snippet: (A) Kaplan-Meier survival curves based on the ITGB6 gene expression level. (B) Kaplan-Meier survival curves based on the Rac1 gene expression level. (C) Receiver Operating Characteristic (ROC) analysis was conducted to predict the prognosis of gastric cancer patients using ITGB6 and Rac1 expression. (D) Receiver Operating Characteristic (ROC) analysis was conducted to predict lymph node metastasis in gastric cancer patients using ITGB6 and Rac1 expression.

Article Snippet: Rabbit polyclonal antibody against ITGB6 (dilution, 1:500; #28378-1-AP; Proteintech; USA) and Rabbit polyclonal antibody against Rac1 (dilution, 1:500; #24072-1-AP; Proteintech; USA) were utilized.

Techniques: Gene Expression, Expressing

Journal: Frontiers in Oncology

Article Title: The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma

doi: 10.3389/fonc.2024.1347270

Figure Lengend Snippet: The correlation between the expression of ITGB6 and Rac1 in human gastric tumor tissues (r =0.285, P <0.001).

Article Snippet: Rabbit polyclonal antibody against ITGB6 (dilution, 1:500; #28378-1-AP; Proteintech; USA) and Rabbit polyclonal antibody against Rac1 (dilution, 1:500; #24072-1-AP; Proteintech; USA) were utilized.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma

doi: 10.3389/fonc.2024.1347270

Figure Lengend Snippet: (A) Survival analysis was conducted on gastric cancer patients in a retrospective cohort, taking into consideration the combined levels of ITGB6 and Rac1. Based on the expression levels of ITGB6 and Rac1, the patients were classified into four groups, and subsequently, survival rates were calculated and represented using Kaplan-Meier curves. (B) In order to predict the prognosis of gastric cancer patients, a Receiver Operating Characteristic (ROC) analysis was performed, utilizing the combined expression levels of ITGB6 and Rac1.

Article Snippet: Rabbit polyclonal antibody against ITGB6 (dilution, 1:500; #28378-1-AP; Proteintech; USA) and Rabbit polyclonal antibody against Rac1 (dilution, 1:500; #24072-1-AP; Proteintech; USA) were utilized.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma

doi: 10.3389/fonc.2024.1347270

Figure Lengend Snippet: Univariate and multivariate analyses applying the Cox proportional hazard model to patients diagnosed with gastric cancer (Retrospective cohort).

Article Snippet: Rabbit polyclonal antibody against ITGB6 (dilution, 1:500; #28378-1-AP; Proteintech; USA) and Rabbit polyclonal antibody against Rac1 (dilution, 1:500; #24072-1-AP; Proteintech; USA) were utilized.

Techniques: Significance Assay

Journal: Frontiers in Oncology

Article Title: The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma

doi: 10.3389/fonc.2024.1347270

Figure Lengend Snippet: (A) Transfection of ITGB6 siRNA was conducted in the SGC7901 gastric cancer cell line, and the transfection efficiency was evaluated using RT-PCR. (B) After inhibiting the expression of ITGB6 in 7901 cells and treating them with the Rac1 activity inhibitor NSC23766, the cell viability was assessed using the CCK8 assays. (C) After interfering with ITGB6 expression in 7901 cells and treating them with the Rac1 activity inhibitor NSC23766, Transwell migration and invasion assays were performed to assess the changes in cell migration and invasion abilities. (D) Transfection of NC and ITGB6 siRNA was performed separately in 7901 cells, followed by treatment with NSC23766. Cell viability was evaluated using the CCK8 assay. (E) In the experiment, we performed co-transfection of NC and ITGB6 siRNA in 7901 cells, accompanied by NSC23766 treatment. The migration and invasion abilities of the cells were assessed through Transwell migration and invasion assays. *P<0.05; **P<0.001; ***P<0.0001

Article Snippet: Rabbit polyclonal antibody against ITGB6 (dilution, 1:500; #28378-1-AP; Proteintech; USA) and Rabbit polyclonal antibody against Rac1 (dilution, 1:500; #24072-1-AP; Proteintech; USA) were utilized.

Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Expressing, Activity Assay, Migration, CCK-8 Assay, Cotransfection